Оптимизация процесса пробоподготовки для профилирования жирных кислот в сложных биологических матрицах
The influence of organic solvent composition on the extraction efficiency of medium- and long-chain fatty acids from human feces flurries has been investigated. To this end we tested the following organic solvents: hexane, methyl tertiary-butyl ether and their mixture in a ratio 1:1. Furthermore, we...
| Published in: | Перспективы развития фундаментальных наук. Т. 2 : сборник научных трудов XX Международной конференции студентов, аспирантов и молодых ученых, 25–28 апреля 2023 г. Т. 2 : Химия. С. 190-192 |
|---|---|
| Main Author: | |
| Format: | Book Chapter |
| Language: | Russian |
| Subjects: | |
| Online Access: | http://vital.lib.tsu.ru/vital/access/manager/Repository/koha:001146739 |
| Summary: | The influence of organic solvent composition on the extraction efficiency of medium- and long-chain fatty acids from human feces flurries has been investigated. To this end we tested the following organic solvents: hexane, methyl tertiary-butyl ether and their mixture in a ratio 1:1. Furthermore, we monitored the effects of ionic strength and pH of the flurries on the recovery of fatty acids. A high-performance liquid chromatography mass spectrometry (HPLS-MS) method was used for monitoring of the fatty acids in the obtained extracts. We report that within the tested conditions the most optimal combination of a recovery and analytical coverage of fatty acids was obtained with flurries containing 4% HCl and 5.5% NaCl and a mixture of methyl tertiary-butyl ether and hexane as an organic phase. |
|---|---|
| Bibliography: | Библиогр.: 12 назв. |
| ISBN: | 9785438711384 9785438711360 |