Оптимизация процесса пробоподготовки для профилирования жирных кислот в сложных биологических матрицах
The influence of organic solvent composition on the extraction efficiency of medium- and long-chain fatty acids from human feces flurries has been investigated. To this end we tested the following organic solvents: hexane, methyl tertiary-butyl ether and their mixture in a ratio 1:1. Furthermore, we...
| Published in: | Перспективы развития фундаментальных наук. Т. 2 : сборник научных трудов XX Международной конференции студентов, аспирантов и молодых ученых, 25-28 апреля 2023 г. Т. 2 : Химия. С. 190-192 |
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| Format: | Book Chapter |
| Language: | Russian |
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| Online Access: | http://vital.lib.tsu.ru/vital/access/manager/Repository/koha:001146739 Перейти в каталог НБ ТГУ |
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| 100 | 1 | |a Сидельников, Владимир Сергеевич |9 478308 | |
| 245 | 1 | 0 | |a Оптимизация процесса пробоподготовки для профилирования жирных кислот в сложных биологических матрицах |c В. С. Сидельников |
| 246 | 1 | 1 | |a Optimization of a sample preparation workflow for profiling of the fatty acids in the complex biological matrices |
| 336 | |a Текст | ||
| 337 | |a электронный | ||
| 504 | |a Библиогр.: 12 назв. | ||
| 520 | 3 | |a The influence of organic solvent composition on the extraction efficiency of medium- and long-chain fatty acids from human feces flurries has been investigated. To this end we tested the following organic solvents: hexane, methyl tertiary-butyl ether and their mixture in a ratio 1:1. Furthermore, we monitored the effects of ionic strength and pH of the flurries on the recovery of fatty acids. A high-performance liquid chromatography mass spectrometry (HPLS-MS) method was used for monitoring of the fatty acids in the obtained extracts. We report that within the tested conditions the most optimal combination of a recovery and analytical coverage of fatty acids was obtained with flurries containing 4% HCl and 5.5% NaCl and a mixture of methyl tertiary-butyl ether and hexane as an organic phase. | |
| 653 | |a метаболизм жирных кислот | ||
| 653 | |a пробоподготовка | ||
| 653 | |a экспериментальные исследования | ||
| 655 | 4 | |a статьи в сборниках |9 975486 | |
| 773 | 0 | |t Перспективы развития фундаментальных наук. Т. 2 : сборник научных трудов XX Международной конференции студентов, аспирантов и молодых ученых, 25-28 апреля 2023 г. |d Томск, 2023 |g Т. 2 : Химия. С. 190-192 |z 9785438711384 |z 9785438711360 | |
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| 856 | |y Перейти в каталог НБ ТГУ |u https://koha.lib.tsu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=1146739 | ||
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