Fluorescent silver clusters on protein templates: understanding their structure
Luminescent metal nanoclusters (NCs) stabilized by natural proteins are of special interest in bioimaging applications. However, the detailed structure of the protein-templated NCs and the nature of their emissive states remain poorly understood. A fair amount of nonluminescent metal ions and cluste...
| Published in: | The Journal of Physical Chemistry C Vol. 122, № 51. P. 29549-29558 |
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| Other Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Online Access: | http://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000650575 Перейти в каталог НБ ТГУ |
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| 008 | 190315|2018 xxu s a eng dd | ||
| 024 | 7 | |a 10.1021/acs.jpcc.8b08306 |2 doi | |
| 035 | |a to000650575 | ||
| 039 | 9 | |a 201903181252 |c 201903151606 |d VLOAD |y 201903151537 |z Александр Эльверович Гилязов | |
| 040 | |a RU-ToGU |b rus |c RU-ToGU | ||
| 245 | 1 | 0 | |a Fluorescent silver clusters on protein templates: understanding their structure |c T. S. Sych, Z. V. Reveguk, V. A. Pomogaev [et al.] |
| 520 | 3 | |a Luminescent metal nanoclusters (NCs) stabilized by natural proteins are of special interest in bioimaging applications. However, the detailed structure of the protein-templated NCs and the nature of their emissive states remain poorly understood. A fair amount of nonluminescent metal ions and clusters complexed to the proteins hinders probing of the structure of the emitting clusters using mass spectroscopy, infrared, or other conventional spectroscopy methods. In this respect, only luminescent excitation spectra distinguish the emitting NCs. In this experimental and theoretical joint study, we modeled the fluorescent excitation and excitation anisotropy spectra of protein-based silver (Ag) NCs. We varied the synthesis conditions and studied the spectral properties of Ag clusters on bovine serum albumin (BSA) and lysozyme, which had already been used as templates, as well as on HMG box (HMGB1) and histone H1 (H1) proteins. We also calculated the electronic spectra of quantum mechanics-optimized Ag-thiolate, Ag-semiquinone, and Ag-formaldehyde complexes with two confined electrons using second-order algebraic diagrammatic construction [ADC(2)] and resolution-of-identity approximate coupled-cluster singles-and-doubles (RI-CC2) methods and compared them with the experimental spectra. We propose a model for the fluorescent Ag-protein complexes in which two reduced Ag atoms are sufficient to form the fluorescent core of the complex. The proposed structural model of the luminescent centers in the Ag-protein complexes differs from the common view that the fluorescent metal NCs in proteins contain about 10 or more metal atoms. The fluorescent Ag clusters formed on the four investigated natural protein matrices exhibited two different spectral and structural patterns. Deprotonated free cysteine residues stabilized the fluorescent Ag3+1 core formed in the BSA matrix. The second type of fluorescent center was realized in the H1, HMGB1, and lysozyme protein matrixes. In this case, tyrosine residues probably stabilize the fluorescent Ag2 centers. | |
| 653 | |a люминесцентные нанокластеры металлов | ||
| 653 | |a серебро | ||
| 653 | |a белковые комплексы | ||
| 655 | 4 | |a статьи в журналах |9 879358 | |
| 700 | 1 | |a Reveguk, Zakhar V. |9 491373 | |
| 700 | 1 | |a Pomogaev, Vladimir A. |9 99172 | |
| 700 | 1 | |a Buglak, Andrey A. |9 491374 | |
| 700 | 1 | |a Reveguk, Anastasiya A. |9 491375 | |
| 700 | 1 | |a Ramazanov, Ruslan R. |9 158980 | |
| 700 | 1 | |a Romanov, Nikolay M. |9 491376 | |
| 700 | 1 | |a Chikhirzhina, Elena V. |9 491377 | |
| 700 | 1 | |a Polyanichko, Alexander M. |9 491378 | |
| 700 | 1 | |a Kononov, Alexei I. |9 491379 | |
| 700 | 1 | |a Sych, Tomash S. |9 491380 | |
| 773 | 0 | |t The Journal of Physical Chemistry C |d 2018 |g Vol. 122, № 51. P. 29549-29558 |x 1932-7447 | |
| 852 | 4 | |a RU-ToGU | |
| 856 | 4 | |u http://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000650575 | |
| 856 | |y Перейти в каталог НБ ТГУ |u https://koha.lib.tsu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=448831 | ||
| 908 | |a статья | ||
| 999 | |c 448831 |d 448831 | ||