Quantitative imaging of white and gray matter remyelination in the cuprizone demyelination model using the macromolecular proton fraction
Macromolecular proton fraction (MPF) has been established as a quantitative clinically-targeted MRI myelin biomarker based on recent demyelination studies. This study aimed to assess the capability of MPF to quantify remyelination using the murine cuprizone-induced reversible demyelination model. MP...
| Published in: | Cells Vol. 8, № 10. P. 1204 (1-18) |
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| Other Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Online Access: | http://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000675550 Перейти в каталог НБ ТГУ |
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| 024 | 7 | |a 10.3390/cells8101204 |2 doi | |
| 035 | |a to000675550 | ||
| 039 | 9 | |a 202002251707 |c 202002141631 |d VLOAD |y 202002141623 |z Александр Эльверович Гилязов | |
| 040 | |a RU-ToGU |b rus |c RU-ToGU | ||
| 245 | 1 | 0 | |a Quantitative imaging of white and gray matter remyelination in the cuprizone demyelination model using the macromolecular proton fraction |c M. Y. Khodanovich, A. O. Pishchelko, V. Y. Glazacheva [et al.] |
| 504 | |a Библиогр.: 72 назв. | ||
| 520 | 3 | |a Macromolecular proton fraction (MPF) has been established as a quantitative clinically-targeted MRI myelin biomarker based on recent demyelination studies. This study aimed to assess the capability of MPF to quantify remyelination using the murine cuprizone-induced reversible demyelination model. MPF was measured in vivo using the fast single-point method in three animal groups (control, cuprizone-induced demyelination, and remyelination after cuprizone withdrawal) and compared to quantitative immunohistochemistry for myelin basic protein (MBP), myelinating oligodendrocytes (CNP-positive cells), and oligodendrocyte precursor cells (OPC, NG2-positive cells) in the corpus callosum, caudate putamen, hippocampus, and cortex. In the demyelination group, MPF, MBP-stained area, and oligodendrocyte count were significantly reduced, while OPC count was significantly increased as compared to both control and remyelination groups in all anatomic structures (p < 0.05). All variables were similar in the control and remyelination groups. MPF and MBP-stained area strongly correlated in each anatomic structure (Pearson's correlation coefficients, r = 0.80-0.90, p < 0.001). MPF and MBP correlated positively with oligodendrocyte count (r = 0.70-0.84, p < 0.01 for MPF; r = 0.81-0.92, p < 0.001 for MBP) and negatively with OPC count (r = -0.69--0.77, p < 0.01 for MPF; r = -0.72--0.89, p < 0.01 for MBP). This study provides immunohistological validation of fast MPF mapping as a non-invasive tool for quantitative assessment of de- and remyelination in white and gray matter and indicates the feasibility of using MPF as a surrogate marker of reparative processes in demyelinating diseases. | |
| 653 | |a магнитно-резонансная томография | ||
| 653 | |a олигодендроциты | ||
| 653 | |a иммуногистохимия | ||
| 653 | |a клетки-предшественники олигодендроцитов | ||
| 653 | |a ремиелинизация | ||
| 653 | |a демиелинизация | ||
| 653 | |a купризоновая модель | ||
| 653 | |a макромолекулярная протонная фракция | ||
| 653 | |a миелин | ||
| 655 | 4 | |a статьи в журналах |9 879358 | |
| 700 | 1 | |a Pishchelko, Anna O. |9 499338 | |
| 700 | 1 | |a Glazacheva, Valentina Y. |9 481831 | |
| 700 | 1 | |a Pan, Edgar S. |9 403912 | |
| 700 | 1 | |a Akulov, Andrey E. |9 405387 | |
| 700 | 1 | |a Svetlik, Mikhail V. |9 853476 | |
| 700 | 1 | |a Tyumentseva, Yana |9 500662 | |
| 700 | 1 | |a Ananina, Tatyana V. |9 97274 | |
| 700 | 1 | |a Yarnykh, Vasily L. |9 102903 | |
| 700 | 1 | |a Khodanovich, Marina Yu. |9 97603 | |
| 773 | 0 | |t Cells |d 2019 |g Vol. 8, № 10. P. 1204 (1-18) |x 2073-4409 | |
| 852 | 4 | |a RU-ToGU | |
| 856 | 4 | |u http://vital.lib.tsu.ru/vital/access/manager/Repository/vtls:000675550 | |
| 856 | |y Перейти в каталог НБ ТГУ |u https://koha.lib.tsu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=463541 | ||
| 908 | |a статья | ||
| 999 | |c 463541 |d 463541 | ||