Разработка и валидация метода количественного определения декскетопрофена трометамола в плазме крови человека
Dexketoprofen (DKP) belongs to the group of nonsteroidal anti-inflammatory drugs exhibiting expressed biological properties. Typically, the concentration of the drug in human plasma is very low, generally in the ng/ml range. Therefore, the aim was to develop and validate a rapid, accurate, precise,...
| Published in: | Перспективы развития фундаментальных наук. Т. 2 : сборник научных трудов XVII Международной конференции студентов, аспирантов и молодых ученых, Россия, Томск, 21-24 апреля 2020 г. Т. 2 : Химия. С. 104-106 |
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| Format: | Book Chapter |
| Language: | Russian |
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| Online Access: | http://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000563620 Перейти в каталог НБ ТГУ |
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| 100 | 1 | |a Лакеев, Александр Павлович |9 487646 | |
| 245 | 1 | 0 | |a Разработка и валидация метода количественного определения декскетопрофена трометамола в плазме крови человека |c А. П. Лакеев |
| 246 | 1 | 1 | |a Development and validation method for quantitative determination of the dexketoprofen trometamol in human plasma |
| 336 | |a Текст | ||
| 337 | |a электронный | ||
| 504 | |a Библиогр.: 4 назв. | ||
| 520 | 3 | |a Dexketoprofen (DKP) belongs to the group of nonsteroidal anti-inflammatory drugs exhibiting expressed biological properties. Typically, the concentration of the drug in human plasma is very low, generally in the ng/ml range. Therefore, the aim was to develop and validate a rapid, accurate, precise, reproducible and highly sensitive HPLC-MS/MS method for the quantitative analysis of DKP in the specified samples. DKP was separated on a Phenomenex Luna С18 column (3 fim, 50^2 mm) at 40°C and analysed in positive-ion electrospray ionization mode. The mobile phase consisted of acetonitrile:0.1% formic acid (60:40, v/v) under isocratic conditions at a flow rate of 0.30 ml/min. The samples were extracted using ethyl acetate with the addition of 0.6 M sulfuric acid and ibuprofen (IBP) as internal standard. Subsequently, the upper organic phase was isolated and 2 il samples were injected onto column for analysis. DKP and IBP eluted at 1.62 ± 0.02 и 2.43 ± 0.03 min, respectively. The total analysis time was 3.5 min. DKP and IBP responses were optimised at the transitions (m/z) 255.2 ^ 105.2 and 206.2 ^ 161.2, respectively. Calibration curves were linear over the range 10-8500 ng/ml with correlation coefficients > 0.999. The method was successfully applied to determine the bioavailability of DKP after a single dose on an empty stomach by volunteers. | |
| 653 | |a декскетопрофен | ||
| 653 | |a плазма крови человека | ||
| 653 | |a нестероидные противовоспалительные препараты | ||
| 653 | |a экспериментальные исследования | ||
| 655 | 4 | |a статьи в сборниках |9 879352 | |
| 773 | 0 | |t Перспективы развития фундаментальных наук. Т. 2 : сборник научных трудов XVII Международной конференции студентов, аспирантов и молодых ученых, Россия, Томск, 21-24 апреля 2020 г. |d Томск, 2020 |g Т. 2 : Химия. С. 104-106 |z 9785868898662 |z 9785868898648 | |
| 852 | 4 | |a RU-ToGU | |
| 856 | 4 | |u http://vital.lib.tsu.ru/vital/access/manager/Repository/koha:000563620 | |
| 856 | |y Перейти в каталог НБ ТГУ |u https://koha.lib.tsu.ru/cgi-bin/koha/opac-detail.pl?biblionumber=563620 | ||
| 908 | |a статья | ||
| 999 | |c 563620 |d 563620 | ||
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